Design thousands of primers instantly using Xpression Primer to facilitate high throughput expression cloning.
Xpression Primer uses a sophisticated algorithm to design thousands of primers in a single search run. The program utilizes statistical optimization techniques to select the best primer pair for each sequence based on your selection criteria. This high-throughput primer design feature is ideal for cloning applications.
In addition to its high-throughput capabilities, Xpression Primer provides comprehensive support to high-throughput cloning systems, including Gateway, BD In-Fusion™, epitope, and TOPO® tools. If you work with other expression systems, the program allows you to add functional tags of your choice and design tagged primers.
The software also offers a sophisticated sequencing primer design feature that can design optimal primers for multiple sequences in a single search run. Plus, you can use Xpression Primer to generate precise transcripts by designing primers to generate sense or antisense transcripts for in vitro expression studies.
Xpression Primer is also web-savvy, allowing you to search Entrez and download batches of ORF sequences directly into the program. And despite its powerful features, Xpression Primer processes three hundred 10kb sequences in approximately 2 minutes.
To use Xpression Primer, you will need an iMac Power PC G3 350 with 256 MB of available RAM and an 800 X 600 screen resolution. The sole limitation is a registration screen that appears at startup and limited functionality.
In summary, Xpression Primer is a fantastic software tool that can revolutionize your primer design process. Its high-throughput capabilities, comprehensive assay support, and sophisticated sequencing primer design feature make it a must-have for anyone involved in expression cloning.
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