ORFprimer is a Java-based tool for automated design of primers. With this software, users can easily and quickly design accurate primers for use in PCR experiments. Its fast and efficient design process saves time and minimizes errors in primer design.
ORFprimer uses the open reading frame information to automatically calculate PCR primers for the sequences. The software's user interface is intuitive and simple, with default settings that make it easy to use. However, users can also customize the calculation options before and after computation of primers, such as shifting the primers position, changing the desired melting temperature, or modifying the restriction sites in the primer sequences.
The adaptiveness and convenience of ORFprimer make it a powerful software tool. The software provides a database interface that allows users to store the retrieved sequences in a local SQL database. MySQL, Oracle, and Microsoft Access are all supported. ORFprimer has numerous features that make it a truly multi-functional software tool.
Some key features of ORFprimer include its simple usage, as it is almost self-explanatory. The graphical user interface (GUI) simplifies the handling of this software. ORFprimer is compatible with different machines, such as Windows, Linux, Mac, and Unix, and it is platform-independent. The Java Web Start Technology allows users to start ORFprimer via the internet, eliminating the need for explicit download and installation procedures.
ORFprimer also features data management tools to help set up a user's own database. Additionally, concurrent access to MySQL, Oracle, and Micosoft Access is supported. Alternatively, data can be written to files in the fasta format. With high throughput computation, ORFprimer can be used to compute primers for thousands of target sequences. This process can be further automated by reading the targets through text files.
Overall, ORFprimer is a comprehensive primer design software that not only simplifies the process but also provides customization options for users. The software's intuitive interface and compatibility with different platforms make it an excellent tool for researchers and scientists alike.
Version 1.6.4.1: N/A